Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. First aid measures general information this solution is not hazardous. Generuler 1 kb plus dna ladder, readytouse thermo fisher. If samples need to be diluted, use te or other buffer of minimal ionic strength. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. The ladder consists of nine bands in even increments 0. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for.
Dna electrophoersis ladder very blurry electrophoresis. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. This product is available in quickload purple format enjoy the convenience of a readytoload format. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp.
The key to accurate band analysis is to use the correct dna ladder for your particular application. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. Pdf merge combine pdf files free tool to merge pdf online. All rna molecules in the ladder are polyatailed 40 nt for convenient use as control templates for cdna synthesis. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp.
Both files must appear in the same folder in drake documents. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. The 1 kb and 3 kb bands contain more dna to provide internal orientation. All include a vial of gel loading dye, purple 6x, no sds. How can i prepare working solution with 1 kb dna ladder. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. How to merge pdfs and combine pdf files adobe acrobat dc. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. View more information about our dna stains technology.
Select or drag your files, then click the merge button to download your document into one pdf file. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. The dna ladder preparation contains, amongst other components, 2 mm trishcl ph 7. Invitrogen 1kb dna extension ladder 100ug nucleic acid. Compare rna ladders from leading suppliers on biocompare. For optimal resolution, the recommended agarose gel concentration is 0. Easily combine multiple files into one pdf document. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. Invitrogen 10787026 view more versions of this product.
The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. There have been no changes to product formulation or specifications. This marker is ideal for the size determination of digested dna. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen egel dna ladders are formulated specifically for maximum performance on invitrogen egel precast agarose gels and are provided in readytoload formats. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments. This format is available for the low molecular weight, 50 bp, 100 bp and 1 kb plus dna ladders. Soda pdf merge tool allows you to combine pdf files in seconds. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular. Nebs quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. All of these form less than 1 % wv of the final mixture, so they are not listed in the table above. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Dna electrophoersis ladder very blurry nov022010 recently my dna reference ladder invitrogen 1kb plus has been giving me a very blurred smeary result, rather than the distinct bands i am used to and used to get. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp.
1286 171 1099 27 795 448 1038 1255 1522 779 356 544 1205 362 1039 121 689 907 850 1351 190 561 134 1432 711 1386 1232 56 907 396 578 1017